Cooperative Ecosystem Studies Unit, Rocky Mountain CESU

Quick Facts

Due DateApril 11, 2014 (Friday)
Award AmountUn-Known
Category of Funding ActivityScience and Technology and other Research and Development
Eligible Applicants
  • Others - This financial assistance opportunity is being issued under a Cooperative Ecosystem Studies Unit (CESU) Program. CESUs are partnerships that provide research, technical assistance, and education. Eligible recipients must be a participating partner of the Rocky Mountain Cooperative Ecosystem Studies Unit (CESU) Program.

Program Description

The US Geological Survey, Wyoming-Montana Water Science Center (WY-MT WSC), is offering a funding opportunity is to provide sample collection, analysis, and interpretation regarding the extraction of genetic information from sediment and water-column samples collected from approximately 12 sites on Lake Powell during two synoptic cruises, CY 2014 and 2015. This work is being done in support of a USGS funded project on mercury cycling in Lake Powell. Specifically, sediment and water column samples will be collected concomitant with those collected for geochemical analyses using sterile techniques. Total nucleic acids will be extracted using a combined physical and chemical lysis method. A set of broad and targeted primer sets will be used to assess the biodiversity of the microbial communities thought to participate in Hg(II-)-methylation. Community biodiversity will be assessed by subjecting genomic DNA to polymerase chain reaction (PCR) using universal primers targeting the V8 hypervariable region within the 16S rRNA gene. The biodiversity of potential methylators (e.g., SRB, IRB, methanogens, firmicutes) will be assessed using primers for the specific amplification of a region of hcgAB, the protein product of which was recently demonstrated to be required for the methylation of Hg. A forward primer specific to the conserved corrinoid binding domain of HcgA and a reverse primer specific to the unique ferredoxin binding domain of HcgB will be developed along with the development of primers for the specific amplification of target functional genes. PCR amplicons will be barcoded and subjected to multiplex pyrosequencing using the GS FLX Titanium platform.

General Information

Funding Opportunity NumberG14AS00049
Funding Opportunity TitleCooperative Ecosystem Studies Unit, Rocky Mountain CESU
Opportunity CategoryScience and Technology and other Research and Development
Funding Instrument TypeCooperative Agreement
Category ExplanationNot Available
Number of Awards1
Cost Sharing or Matching RequirementNo
Posted DateApril 2, 2014 (Wednesday)
Application Due DateApril 11, 2014 (Friday)
Application Archive DateJuly 2, 2014 (Wednesday)
Total Program Funding$ 12,840
Award AmountUn-Known
CFDA Number
  • 15.808 - U.S. Geological Survey_ Research and Data Collection

Eligible Applicants

  • Others - This financial assistance opportunity is being issued under a Cooperative Ecosystem Studies Unit (CESU) Program. CESUs are partnerships that provide research, technical assistance, and education. Eligible recipients must be a participating partner of the Rocky Mountain Cooperative Ecosystem Studies Unit (CESU) Program.

Contact Information

Agency NameDepartment of the Interior
Program Information Websitehttp://www.grants.gov/
ContactOffice: Geological Survey
Faith Graves, 703-648-7356
fgraves@usgs.gov

fgraves@usgs.gov

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